Composite

Part:BBa_K3733040

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-10-01)


Antimicrobial peptide LTA with signal peptide and affinity tags

LTA is a novel antimicrobial peptide (AMP), whose design was based on combing the active centers of a ride range of AMPs, including LL-37, YW12D, innate defense regulator 1, and cathelicidin 2 with thymopentin or the active center of thymosin alpha 1 (Tα1). It could neutralize Lipopolysaccharides (LPS), thus effectively blocking the downstream inflammation pathway. With the 6xHis placed after LTA, it then can be recovered in high purified form by changing the elution condition to release the protein from the chromatography column. PhoA, a signal peptide of Sec pathway in Escherichia coli, have the capability to guide the LTA excrete out of the cell.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None